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- #1 Microinjection of mRNA into fertilized or cloned zygotes
Overexpress a gene of interest by microinjection of mRNA into fertilized or cloned zygotes.
- #2 Collection of mouse spermatogenic cells
Spermatogenic cells for microinjection can be collected from the testes by a mechanical or chemical (enzyme) treatment.
- Cryopreservation of mouse embryos
A video protocol of embryo vitrification broadly applicable to different strains of mice. It is routinely used at RIKEN BRC.
- Somatic cell nuclear transfer (SCNT)
A standard protocol for mouse SCNT using Piezo-assisted intracytoplasmic injection