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#1 Microinjection of mRNA into fertilized or cloned zygotes PDF(368KB)

Overexpress a gene of interest by microinjection of mRNA into fertilized or cloned zygotes.

#2 Collection of mouse spermatogenic cells PDF(368KB)

Spermatogenic cells for microinjection can be collected from the testes by a mechanical or chemical (enzyme) treatment.

Cryopreservation of mouse embryos

A video protocol of embryo vitrification broadly applicable to different strains of mice. It is routinely used at RIKEN BRC.

Somatic cell nuclear transfer (SCNT)

A standard protocol for mouse SCNT using Piezo-assisted intracytoplasmic injection