{"id":604,"date":"2020-08-06T11:09:19","date_gmt":"2020-08-06T02:09:19","guid":{"rendered":"http:\/\/kougaku.brc.riken.jp\/ja\/?page_id=604"},"modified":"2025-08-20T13:53:42","modified_gmt":"2025-08-20T04:53:42","slug":"r2019","status":"publish","type":"page","link":"http:\/\/kougaku.brc.riken.jp\/ja\/research\/research-bed\/r2019","title":{"rendered":"\u7814\u7a76\u5185\u5bb9 2019\u5e74\u7248"},"content":{"rendered":"
\n

\u30df\u30c3\u30b7\u30e7\u30f3\u3068\u6982\u8981<\/h2>\n

\u5f53\u7814\u7a76\u5ba4\u3067\u306f\u3001BRC\u304c\u5404\u30ea\u30bd\u30fc\u30b9\u3001\u7279\u306b\u5b9f\u9a13\u52d5\u7269\u304a\u3088\u3073\u5e79\u7d30\u80de\u3092\u3088\u308a\u9ad8\u5ea6\u306a\u30af\u30aa\u30ea\u30c6\u30a3\u306b\u3066\u7dad\u6301\u4f9b\u7d66\u3059\u308b\u305f\u3081\u306b\u5fc5\u8981\u306a\u907a\u4f1d\u95a2\u9023\u6280\u8853\u958b\u767a\u3092\u884c\u3046\u3002
\n \u307e\u305f\u3001\u3053\u308c\u3089\u306e\u6280\u8853\u304c\u5e83\u304f\u7814\u7a76\u30b3\u30df\u30e5\u30cb\u30c6\u30a3\u306b\u6d3b\u7528\u3055\u308c\u308b\u3079\u304f\u3001\u7814\u4fee\u4e8b\u696d\u3092\u884c\u3046\u3002<\/p>\n

<\/p>\n

\u6280\u8853\u958b\u767a<\/h2>\n
    \n
  1. \u4f53\u7d30\u80de\u6838\u79fb\u690d\u30af\u30ed\u30fc\u30f3\u6280\u8853\u306e\u958b\u767a<\/a><\/li>\n
  2. \u9855\u5fae\u6388\u7cbe\u6280\u8853\u306e\u958b\u767a<\/a><\/li>\n
  3. \u52b9\u7387\u7684\u306a\u80da\u30fb\u914d\u5076\u5b50\u306e\u51cd\u7d50\u4fdd\u5b58\u6cd5\u306e\u958b\u767a<\/a><\/li>\n
  4. \u65b0\u898f\u5e79\u7d30\u80de\u304a\u3088\u3073\u65b0\u898f\u52d5\u7269\u30e2\u30c7\u30eb\u306e\u958b\u767a<\/a><\/li>\n
  5. \u6280\u8853\u7814\u4fee<\/a><\/li>\n<\/ol>\n

    \u7814\u7a76\u5185\u5bb9\u30002019\u5e74\u7248<\/h2>\n

    1.\u4f53\u7d30\u80de\u6838\u79fb\u690d\u30af\u30ed\u30fc\u30f3\u6280\u8853\u306e\u958b\u767a<\/a><\/h3>\n

    \u4f53\u7d30\u80de\u6838\u79fb\u690d\u306b\u3088\u308b\u30af\u30ed\u30fc\u30f3\u6280\u8853\u306f\u3001\u5e0c\u5c11\u306a\u7cfb\u7d71\u306e\u4fdd\u5b58\u304a\u3088\u3073\u65b0\u898f\u30e2\u30c7\u30eb\u52d5\u7269\u4f5c\u51fa\u306a\u3069\u306b\u6709\u7528\u306a\u6280\u8853\u3067\u3042\u308b\u304c\u3001\u672a\u3060\u306b\u30af\u30ed\u30fc\u30f3\u500b\u4f53\u306e\u4f5c\u51fa\u52b9\u7387\u306f\u4f4e\u304f\u3001\u6280\u8853\u7684\u6539\u5584\u304c\u5fc5\u8981\u3067\u3042\u308b\u3002\u3053\u308c\u307e\u3067\u6211\u3005\u306f\u3001\u30de\u30a6\u30b9\u3092\u30e2\u30c7\u30eb\u3068\u3057\u3066\u69d8\u3005\u306a\u7d30\u80de\u7a2e\u30fb\u7cfb\u7d71\u304b\u3089\u30af\u30ed\u30fc\u30f3\u500b\u4f53\u3092\u4f5c\u51fa\u3057\u3066\u304d\u305f\u3002\u305d\u306e\u4e00\u65b9\u3067\u3001\u5fae\u5c0f\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u306e\u907a\u4f1d\u5b50\u767a\u73fe\u304a\u3088\u3073\u30a8\u30d4\u30b2\u30ce\u30e0\u89e3\u6790\u6280\u8853\u3092\u5fdc\u7528\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u30af\u30ed\u30fc\u30f3\u80da\u3067\u8d77\u3053\u308b\u69d8\u3005\u306a\u30a8\u30d4\u30b2\u30ce\u30e0\u7570\u5e38\u3092\u540c\u5b9a\u3057\u3066\u304d\u305f\u3002<\/p>\n

    \u4ee3\u8868\u7684\u306a\u907a\u4f1d\u5b50\u767a\u73fe\u30fb\u30a8\u30d4\u30b2\u30ce\u30e0\u7570\u5e38\u3068\u3057\u3066\u3001\u672c\u6765X\u67d3\u8272\u4f53\u304b\u3089\u8d77\u3053\u308b\u907a\u4f1d\u5b50\u767a\u73fe\u304c\u30af\u30ed\u30fc\u30f3\u80da\u3067\u306f\u5927\u898f\u6a21\u306b\u6291\u5236\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u3001\u305d\u3057\u3066\u3053\u308c\u304cXist<\/i>\u907a\u4f1d\u5b50\u306e\u7570\u5e38\u306a\u6d3b\u6027\u5316\u306b\u8d77\u56e0\u3059\u308b\u3053\u3068\u3092\u767a\u898b\u3057\u305f(Inoue et al., Science<\/i>, 2010)\u3002\u3053\u306eXist<\/i>\u306e\u904e\u5270\u767a\u73fe\u306f\u3001Xist<\/i>\u306e\u907a\u4f1d\u7684\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u3082\u3057\u304f\u306fsiRNA\u306b\u3088\u308b\u30ce\u30c3\u30af\u30c0\u30a6\u30f3\u3067\u88dc\u6b63\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u3001\u305d\u306e\u7d50\u679c9-10%\u7a0b\u5ea6\u307e\u3067\u30af\u30ed\u30fc\u30f3\u500b\u4f53\u306e\u4f5c\u51fa\u52b9\u7387\u3092\u4e0a\u3052\u308b\u3053\u3068\u306b\u6210\u529f\u3057\u305f\u3002(Inoue et al., Science<\/i>, 2010; Matoba et al., PNAS<\/i>, 2011)\u3002
    \n\u307e\u305f\u3001\u30af\u30ed\u30fc\u30f3\u80da\u306e\u521d\u671f\u767a\u751f\u963b\u5bb3\u56e0\u5b50\u3068\u3057\u3066\u30d2\u30b9\u30c8\u30f3H3\u306e9\u756a\u76ee\u30ea\u30b8\u30f3\u306e\u30c8\u30ea\u30e1\u30c1\u30eb\u5316(H3K9me3)\u3092\u540c\u5b9a\u3057\u305f(Matoba et al., Cell<\/i>, 2014)\u3002
    \n\u3053\u306eH3K9me3\u306f\u30d2\u30b9\u30c8\u30f3\u8131\u30e1\u30c1\u30eb\u5316\u9175\u7d20\u3067\u3042\u308bKdm4\u3092\u30af\u30ed\u30fc\u30f3\u80da\u3067\u767a\u73fe\u3055\u305b\u308b\u3053\u3068\u3067\u9664\u53bb\u3067\u304d\u3001\u305d\u306e\u7d50\u679c\u30af\u30ed\u30fc\u30f3\u500b\u4f53\u306e\u4f5c\u51fa\u52b9\u7387\u30928-9%\u7a0b\u5ea6\u307e\u3067\u5927\u5e45\u306b\u6539\u5584\u3059\u308b\u3053\u3068\u306b\u6210\u529f\u3057\u305f(Matoba et al., Cell<\/i>, 2014)\u3002
    \n\u3055\u3089\u306b\u6700\u8fd1\u3067\u306f\u3001Xist<\/i>\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u88dc\u6b63\u3068Kdm4\u306b\u3088\u308bH3K9me3\u306e\u9664\u53bb\u6280\u8853\u3092\u7d44\u307f\u5408\u308f\u305b\u308b\u3053\u3068\u3067\u3001\u30de\u30a6\u30b9\u306e\u30af\u30ed\u30fc\u30f3\u4f5c\u51fa\u52b9\u7387\u3092\u6700\u592723%\u306b\u307e\u3067\u9ad8\u3081\u308b\u3053\u3068\u306b\u6210\u529f\u3057\u3066\u3044\u308b(Matoba et al., Cell Stem Cell<\/i>, 2018)\u3002<\/p>\n

    \u3057\u304b\u3057\u306a\u304c\u3089\u3001\u3053\u306e\u3088\u3046\u306a\u6700\u9069\u5316\u3057\u305f\u30af\u30ed\u30fc\u30f3\u80da\u3067\u3082\u307e\u3060\u53d7\u7cbe\u80da\u3068\u6bd4\u3079\u308b\u3068\u500b\u4f53\u306e\u4f5c\u51fa\u52b9\u7387\u306f\u534a\u5206\u4ee5\u4e0b\u3068\u4f4e\u304f\u3001\u3055\u3089\u306b\u30af\u30ed\u30fc\u30f3\u80da\u7279\u6709\u306e\u80ce\u76e4\u306e\u5f62\u6210\u7570\u5e38\u304c\u8a8d\u3081\u3089\u308c\u308b\u3002
    \n\u6700\u8fd1\u6211\u3005\u306f\u305d\u306e\u539f\u56e0\u306e\u5019\u88dc\u3068\u3057\u3066\u3001\u6bcd\u6027H3K27me3\u306b\u3088\u308b\u30b2\u30ce\u30e0\u30a4\u30f3\u30d7\u30ea\u30f3\u30c6\u30a3\u30f3\u30b0\u306e\u55aa\u5931\u304c\u95a2\u4e0e\u3057\u3066\u3044\u308b\u53ef\u80fd\u6027\u3092\u898b\u51fa\u3057\u305f(\u56f31, Okae et al., Hum Mol Genet<\/i>, 2014; Hirose et al., Epigenetics<\/i>, 2018; Matoba et al., Cell Stem Cell<\/i>, 2018)\u3002
    \n\u901a\u5e38\u53d7\u7cbe\u80da\u3067\u306f\u6bcd\u65b9\u7531\u6765\u306e\u907a\u4f1d\u5b50\u306e\u4e00\u90e8\u304cH3K27me3\u306b\u3088\u3063\u3066\u6291\u5236\u3055\u308c\u3066\u304a\u308a\u3001\u305d\u306e\u7d50\u679c\u3068\u3057\u3066\u7236\u65b9\u767a\u73fe\u3092\u793a\u3059\u304c\u3001\u30af\u30ed\u30fc\u30f3\u80da\u3067\u306fH3K27me3\u306b\u3088\u308b\u6291\u5236\u304c\u5b58\u5728\u305b\u305a\u3001\u4e21\u30a2\u30ec\u30eb\u304b\u3089\u904e\u5270\u306b\u767a\u73fe\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u3063\u305f(Matoba et al., Cell Stem Cell<\/i>, 2018)\u3002
    \n\u3053\u306e\u3088\u3046\u306a\u7570\u5e38\u3092\u793a\u3059\u30a4\u30f3\u30d7\u30ea\u30f3\u30c8\u907a\u4f1d\u5b50\u306f\u3001\u591a\u304f\u304c\u80ce\u76e4\u306e\u5f62\u6210\u306b\u95a2\u4e0e\u3057\u3066\u3044\u308b\u3002
    \n\u6700\u8fd1\u6211\u3005\u306f\u305d\u308c\u3089\u306e\u907a\u4f1d\u5b50\u306e\u4e00\u90e8\u3067\u3042\u308bSfmbt2<\/i> \u30de\u30a4\u30af\u30ed (mi) RNA\u3084Slc38a4<\/i>\u304c\u6b63\u5e38\u306a\u80ce\u76e4\u5f62\u6210\u306b\u5fc5\u9808\u3067\u3042\u308b\u3053\u3068\u3092\u793a\u3057\uff08Inoue et al., Cell Rep<\/i>., 2017; Matoba et al., PNAS<\/i>, 2019\uff09\u3001\u305d\u306e\u5185Sfmbt2<\/i> miRNA\u306e\u767a\u73fe\u88dc\u6b63\u304c\u30af\u30ed\u30fc\u30f3\u80da\u306e\u80ce\u76e4\u5f62\u6210\u7570\u5e38\u3092\u6539\u5584\u3059\u308b\u3053\u3068\u3092\u660e\u3089\u304b\u306b\u3057\u305f\uff08\u56f32, Inoue et al., in revision\uff09\u3002
    \n\u4eca\u5f8c\u3082H3K27me3\u306b\u3088\u308b\u30a4\u30f3\u30d7\u30ea\u30f3\u30c8\u907a\u4f1d\u5b50\u7fa4\u306e\u30af\u30ed\u30fc\u30f3\u80da\u80ce\u76e4\u5f62\u6210\u306b\u5bfe\u3059\u308b\u95a2\u4e0e\u3092\u89e3\u660e\u3059\u308b\u3053\u3068\u3067\u3001\u30af\u30ed\u30fc\u30f3\u80da\u306e\u4f5c\u88fd\u52b9\u7387\u304a\u3088\u3073\u54c1\u8cea\uff08\u7279\u306b\u80ce\u76e4\uff09\u3092\u6539\u5584\u3059\u308b\u3053\u3068\u304c\u671f\u5f85\u3055\u308c\u308b\u3002
    \n\u3055\u3089\u306b\u3001\u30c9\u30ca\u30fc\u7d30\u80de\u3067\u306e\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u3068\u7d44\u307f\u5408\u308f\u305b\u308b\u3053\u3068\u3067\u3001\u30af\u30ed\u30fc\u30f3\u6280\u8853\u306f\u533b\u5b66\u751f\u7269\u5b66\u7814\u7a76\u306b\u6709\u7528\u306a\u5b9f\u9a13\u52d5\u7269\u30e2\u30c7\u30eb\u306e\u4f5c\u51fa\u306b\u3055\u3089\u306b\u8ca2\u732e\u3067\u304d\u308b\u3068\u8003\u3048\u3089\u308c\u308b\u3002<\/p>\n

    \"\u56f31\uff1a\u30af\u30ed\u30fc\u30f3\u80da\u80ce\u76e4\u306b\u304a\u3051\u308bH3K27me3\u30b2\u30ce\u30e0\u30a4\u30f3\u30d7\u30ea\u30f3\u30c6\u30a3\u30f3\u30b0\u306e\u55aa\u5931\"
    \n \u56f31\uff1a\u30af\u30ed\u30fc\u30f3\u80da\u80ce\u76e4\u306b\u304a\u3051\u308bH3K27me3\u30b2\u30ce\u30e0\u30a4\u30f3\u30d7\u30ea\u30f3\u30c6\u30a3\u30f3\u30b0\u306e\u55aa\u5931<\/p>\n

    <\/p>\n

    \"\u56f32\uff1a\u53d7\u7cbe\u80da\u3001\u91ce\u751f\u578b\u30af\u30ed\u30fc\u30f3\u80da\u3001Sfmbt2
    \n \u56f32\uff1a\u53d7\u7cbe\u80da\u3001\u91ce\u751f\u578b\u30af\u30ed\u30fc\u30f3\u80da\u3001Sfmbt2 miRNA KO\u30af\u30ed\u30fc\u30f3\u80da\u306e\u80ce\u76e4\u5f62\u614b<\/p>\n

    2.\u9855\u5fae\u6388\u7cbe\u6280\u8853\u306e\u958b\u767a<\/a><\/h3>\n

    \u4e00\u6b21\u7cbe\u6bcd\u7d30\u80de\u3092\u7528\u3044\u305f\u9855\u5fae\u6388\u7cbe\u306f\u3001\u3059\u3067\u306b\u7523\u4ed4\u7372\u5f97\u306e\u5831\u544a\u304c\u3055\u308c\u3066\u3044\u308b\u304c\u3001\u305d\u306e\u6210\u529f\u7387\u306f4\uff05\u4ee5\u4e0b\u3068\u3001\u521d\u671f\u306e\u30de\u30a6\u30b9\u4f53\u7d30\u80de\u30af\u30ed\u30fc\u30f3\u6210\u529f\u7387\u306b\u5339\u6575\u3059\u308b\u307b\u3069\u4f4e\u7387\u3067\u3042\u308b (Ogura et al., PNAS<\/i>, 1998; Miki et al., J Reprod Dev<\/i>, 2006)\u3002
    \n\u305d\u306e\u539f\u56e0\u3068\u3057\u3066\u3001\u4e00\u6b21\u7cbe\u6bcd\u7d30\u80de\u306e\u67d3\u8272\u4f53\u304c\u5375\u5b50\u5185\u3067\u6e1b\u6570\u5206\u88c2\u3092\u9032\u884c\u3059\u308b\u969b\u3001\u67d3\u8272\u4f53\u5206\u96e2\u30a8\u30e9\u30fc\u304c\u9ad8\u983b\u5ea6\u306b\u751f\u3058\u308b\u3053\u3068\u304c\u6319\u3052\u3089\u308c\u3066\u3044\u308b\u3002
    \n\u305d\u3053\u3067\u3001\u7406\u5316\u5b66\u7814\u7a76\u6240\u751f\u547d\u6a5f\u80fd\u79d1\u5b66\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u306e\u4eac\u6975\u535a\u4e45\u3089(Dev Cell 2017)\u304c\u5831\u544a\u3057\u305f\u300e\u7d30\u80de\u8cea\u30b5\u30a4\u30ba\u3092\u5c0f\u3055\u304f\u3057\u305f\u5375\u5b50\u300f\u306e\u3001\u7d21\u9318\u4f53\u6975\u306e\u5b89\u5b9a\u5316\u30fb\u7d21\u9318\u4f53\u30c1\u30a7\u30c3\u30af\u30dd\u30a4\u30f3\u30c8\u306e\u6b63\u78ba\u5316\u3092\u5229\u7528\u3057\u3066\u3001\u4e00\u6b21\u7cbe\u6bcd\u7d30\u80de\u306e\u9855\u5fae\u6ce8\u5165\u5375\u3068\u3057\u3066\u7528\u3044\u305f\u3068\u3053\u308d\u3001
    \n\u4e00\u6b21\u7cbe\u6bcd\u7d30\u80de\u7531\u6765\u67d3\u8272\u4f53\u306e\u7d21\u9318\u4f53\u8d64\u9053\u9762\u3078\u306e\u6574\u5217\u306e\u6b63\u78ba\u6027\u304c\u5411\u4e0a\u3057\u3001\u305d\u306e\u5f8c\u306e\u67d3\u8272\u4f53\u5206\u96e2\u30a8\u30e9\u30fc\u306e\u6e1b\u5c11\u304c\u67d3\u8272\u4f53\u6a19\u672c\u3088\u308a\u78ba\u8a8d\u3055\u308c\u305f\uff08\u4e0b\u8868\u304a\u3088\u3073\u56f3\uff09\u3002
    \n\u64cd\u4f5c\u80da\u3092\u30ec\u30b7\u30d4\u30a8\u30f3\u30c8\u30de\u30a6\u30b9\u306b\u80da\u79fb\u690d\u3057\u305f\u7d50\u679c\u300120-30\uff05\u306e\u7523\u4ed4\u7387\u304c\u8a8d\u3081\u3089\u308c\u305f\uff08\u65ed\u5ddd\u533b\u5927\u65e5\u91ce\u654f\u662d\u5148\u751f\u3068\u306e\u5171\u540c\u7814\u7a76\uff09\u3002<\/p>\n

    \u8868\uff1a\u4e00\u6b21\u7cbe\u6bcd\u7d30\u80de\u6ce8\u5165\u80da\u306e\u67d3\u8272\u4f53\u89e3\u6790
    \n \"\u8868\uff1a\u4e00\u6b21\u7cbe\u6bcd\u7d30\u80de\u6ce8\u5165\u80da\u306e\u67d3\u8272\u4f53\u89e3\u6790<\"<\/p>\n

    \u56f3\uff1a\u7d30\u80de\u8cea\u30b5\u30a4\u30ba\u3092\u5c0f\u3055\u304f\u3057\u305f\u5375\u5b50\u3092\u7528\u3044\u305f\u4e00\u6b21\u7cbe\u6bcd\u7d30\u80de\u6ce8\u5165\u80da\u306e\u67d3\u8272\u4f53\u89e3\u6790
    \n \"\u56f3\uff1a\u7d30\u80de\u8cea\u30b5\u30a4\u30ba\u3092\u5c0f\u3055\u304f\u3057\u305f\u5375\u5b50\u3092\u7528\u3044\u305f\u4e00\u6b21\u7cbe\u6bcd\u7d30\u80de\u6ce8\u5165\u80da\u306e\u67d3\u8272\u4f53\u89e3\u6790\"
    \n \"\"<\/p>\n

    3.\u52b9\u7387\u7684\u306a\u80da\u30fb\u914d\u5076\u5b50\u306e\u51cd\u7d50\u4fdd\u5b58\u6cd5\u306e\u958b\u767a<\/a><\/h3>\n

    \u80da\u51cd\u7d50\u304c\u56f0\u96e3\u306a\u91ce\u751f\u7531\u6765\u306e\u7570\u7a2e\u30de\u30a6\u30b9(M. spicilegus)\u3067\u3042\u308bZBN\/Ms\u3068SPI\/TUA\u306e2\u7cfb\u7d71\u304b\u3089\u6a39\u7acb\u3057\u305fES\u7d30\u80de\u3092ICR\u306e8\u7d30\u80de\u671f\u80da\u304a\u3088\u3073\u80da\u76e4\u80de\u3078\u6ce8\u5165\u3057\u305f\u7d50\u679c\u3001\u7570\u7a2e\u9593\u30ad\u30e1\u30e9\u30de\u30a6\u30b9\u306e\u4f5c\u51fa\u306b\u6210\u529f\u3057\u305f\u3002\u73fe\u5728\u3001\u4ea4\u914d\u5b9f\u9a13\u3092\u9032\u3081\u3066\u304a\u308a\u3001ES\u7d30\u80de\u7531\u6765\u306e\u7523\u5b50\u304c\u5f97\u3089\u308c\u308c\u3070\u3001ES\u7d30\u80de\u306b\u3088\u308b\u7570\u7a2e\u30de\u30a6\u30b9\u7cfb\u7d71\u306e\u9577\u671f\u4fdd\u5b58\u304c\u53ef\u80fd\u3068\u306a\u308b\u3002<\/p>\n

    \"\u56f3\uff11\uff1aZBNN\/Ms\u304b\u3089\u6a39\u7acb\u3057\u305fES\u7d30\u80de\u7531\u6765\u306e\u30ad\u30e1\u30e9\u30de\u30a6\u30b9\u3068SPI\/TUA\u304b\u3089\u6a39\u7acb\u3057\u305fES\u7d30\u80de\u7531\u6765\u306e\u30ad\u30e1\u30e9\u30de\u30a6\u30b9\"<\/p>\n

    \u904e\u6392\u5375\u3068\u80da\u767a\u751f\u304c\u56f0\u96e3\u306a\u8fd1\u4ea4\u7cfb\u30de\u30a6\u30b9\u306e\u4ee3\u8868\u3068\u3055\u308c\u308bA\u7cfb\u7d71\u3067\u3001\u30d7\u30ed\u30b2\u30b9\u30c6\u30ed\u30f3\u6295\u4e0e\u306b\u3088\u308b\u6027\u5468\u671f\u540c\u671f\u5316\u3068\u6297\u30a4\u30f3\u30d2\u30d3\u30f3\u8840\u6e05\u6295\u4e0e\u3092\u7d44\u307f\u5408\u308f\u305b\u305f\u904e\u6392\u5375\u6cd5 (Hasegawa et al., Biol Reprod<\/i>, 2016)\u306b\u3088\u308a\u3001\u6b63\u5e38\u5375\u5b50\u6570\u304c17\u500b\u304b\u308952\u500b\u306b\u5897\u52a0\u3057\u305f\u3002\u3055\u3089\u306b\u4f53\u5916\u53d7\u7cbe\u5f8c\u306b\u524d\u6838\u671f\u3067\u80da\u79fb\u690d\u3092\u884c\u3044\u4f53\u5185\u30672\u7d30\u80de\u671f\u306b\u767a\u751f\u3057\u305f\u80da\u3092\u30ac\u30e9\u30b9\u5316\u4fdd\u5b58\u3059\u308b\u3053\u3068\u3067\u80da\u79fb\u690d\u5f8c\u306e\u7523\u5b50\u6570\u3092\u6539\u5584\u3067\u304d\u3001
    \n\u96cc1\u5339\u304b\u3089\u306e\u7523\u5b50\u7372\u5f97\u52b9\u7387\u30921.7\u5339\u304b\u308915.1\u5339\u306b\u5897\u52a0\u3067\u304d\u305f\u3002<\/p>\n

    \"\u56f3\uff12\uff1a\u6392\u5375\u6570\u304a\u3088\u3073\u53d7\u7cbe\u7387\u306e\u6bd4\u8f03\u3000\u8868\uff11\uff1a\u51cd\u7d50\u80da\u306e\u79fb\u690d\u6210\u7e3e\"<\/p>\n

    4.\u65b0\u898f\u5e79\u7d30\u80de\u304a\u3088\u3073\u65b0\u898f\u52d5\u7269\u30e2\u30c7\u30eb\u306e\u958b\u767a<\/a><\/h3>\n

    \u6804\u990a\u819c\u5e79\u7d30\u80de (trophoblast stem cell, TS\u7d30\u80de) \u306f\u80da\u76e4\u80de\u306e\u6804\u990a\u5916\u80da\u8449\u304b\u3089\u6a39\u7acb\u3055\u308c\u308b\u672a\u5206\u5316\u306a\u80ce\u76e4\u7cfb\u5217\u306e\u57f9\u990a\u5e79\u7d30\u80de\u3067\u3042\u308a\u3001\u80da\u767a\u751f\u306e\u7814\u7a76\u306b\u6709\u7528\u306a\u30c4\u30fc\u30eb\u3067\u3042\u308b\u3002
    \n\u5f53\u7814\u7a76\u5ba4\u306f\u904e\u53bb\u306bTS\u7d30\u80de\u304c\u672a\u5206\u5316\u6027\u306e\u7570\u306a\u308b\u4e0d\u5747\u4e00\u306a\u96c6\u56e3\u304b\u3089\u69cb\u6210\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u3092\u660e\u3089\u304b\u306b\u3057(Motomura et al., Biol Reprod.<\/i>, 2016)\u3001TS\u7d30\u80de\u306e\u54c1\u8cea\u3092\u5b89\u5b9a\u5316\u3055\u305b\u308b\u305f\u3081\u306e\u6280\u8853\u6539\u826f\u304c\u5fc5\u8981\u3067\u3042\u308b\u3053\u3068\u3092\u793a\u3057\u3066\u304d\u305f\u3002
    \n\u73fe\u5728\u3001\u30d2\u30b9\u30c8\u30f3H3\u306e\u30d0\u30ea\u30a2\u30f3\u30c8\u3001H3.1\u3068H3.2 (=H3.1\/2) \u304cTS\u7d30\u80de\u306eidentity\u306e\u78ba\u7acb\u306b\u91cd\u8981\u3067\u3042\u308b\u53ef\u80fd\u6027\u304c\u5f97\u3089\u308c\u3066\u3044\u308b\u305f\u3081\u3001\u305d\u306e\u8a73\u3057\u3044\u89e3\u6790\u3092\u884c\u3063\u3066\u3044\u308b\u3002
    \n\u5177\u4f53\u7684\u306b\u3001H3.1\/2\u304cH3K9me3\u3092\u5236\u5fa1\u3059\u308b\u3053\u3068\u3067TS\u7d30\u80de\u306b\u7279\u7570\u7684\u306a\u30af\u30ed\u30de\u30c1\u30f3\u69cb\u9020\u3092\u5f62\u6210\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u5206\u304b\u3063\u305f (\u56f31)\u3002\u66f4\u306bTS\u7d30\u80de\u306b\u304a\u3044\u3066\u3001H3.1\/2\u3092\u30af\u30ed\u30de\u30c1\u30f3\u306b\u7d44\u307f\u8fbc\u3080\u56e0\u5b50\u3067\u3042\u308bP150\u3092\u6b20\u640d\u3055\u305b\u305f\u3068\u3053\u308d\u3001TS\u7d30\u80de\u306e\u30de\u30fc\u30ab\u30fc\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u91cf\u304c\u6e1b\u5c11\u3057\u3001ES\u7d30\u80de\u306e\u30de\u30fc\u30ab\u30fc\u907a\u4f1d\u5b50\u3067\u3042\u308bOct3\/4\u306e\u767a\u73fe\u91cf\u304c\u5897\u52a0\u3057\u305f\u3002\u3053\u308c\u3089\u306e\u7d50\u679c\u304b\u3089\u3001H3.1\/2\u3092\u5236\u5fa1\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u3001\u3088\u308a\u8cea\u306e\u9ad8\u3044TS\u7d30\u80de\u3092\u7dad\u6301\u3067\u304d\u308b\u53ef\u80fd\u6027\u3092\u8003\u3048\u3066\u3044\u308b\u3002<\/p>\n

    \"\u56f3\uff11\uff1aTS\u7d30\u80de\u306b\u304a\u3044\u3066H3.1\/2\u306f\u5927\u304d\u306a\u30c9\u30e1\u30a4\u30f3\u3092\u5f62\u6210\u3059\u308b\u3002\"<\/p>\n

    \u6628\u5e74\u5ea6\u306b\u5f15\u304d\u7d9a\u304d\u3001\u30de\u30a6\u30b9\u3067\u660e\u3089\u304b\u306b\u3055\u308c\u3066\u3044\u306a\u3044\u907a\u4f1d\u5b50\u306e\u6a5f\u80fd\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u305f\u3081\u306b\u3001in vivo CRISPR\/Cas9\uff08GONAD\u6cd5\uff09\uff08\u56f3\uff09\u306b\u3088\u308b\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30cf\u30e0\u30b9\u30bf\u30fc\u4f5c\u51fa\u3092\u884c\u3044 (Hirose et al., PNAS<\/i>, 2020)\u3001 ATP \u611f\u53d7\u6027\u30ab\u30ea\u30a6\u30e0\u30c1\u30e3\u30cd\u30eb\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8Kir6.2\u306e\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u306b\u6210\u529f\u3057\u305f\u3002
    \n\u4eca\u5f8c\u3001\u30db\u30e2\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u500b\u4f53\u306e\u7cd6\u4ee3\u8b1d\u3092\u89e3\u6790\u3059\u308b\u4e88\u5b9a\u3067\u3042\u308b\u3002<\/p>\n

    \"\"<\/p>\n

    5.\u6280\u8853\u7814\u4fee<\/a><\/h3>\n

    2016\u5e74\u5ea6\u304b\u30892019\u5e74\u5ea6\u306b\u304a\u3044\u3066\u3001\u6240\u5916\u304b\u3089\u306e\u7814\u4fee\u751f\u3092\u5bfe\u8c61\u306b\u4ee5\u4e0b\u306e\u7814\u4fee\u3092\u884c\u3063\u305f\u3002<\/p>\n

      \n
    • 2016\u5e7410\u6708 \u300c\u30de\u30a6\u30b9\u7cbe\u5b50\u30fb\u80da\u306e\u51cd\u7d50\u4fdd\u5b58\u65b9\u6cd5\u306b\u95a2\u3059\u308b\u6280\u8853\u7814\u4fee
      \n \uff5eBRC\u65b0\u904e\u6392\u5375\u6cd5\u3068\u6b21\u4e16\u4ee3\u30de\u30a6\u30b9\u306e\u65e9\u671f\u4f5c\u51fa\u6cd5\uff5e\u300d<\/li>\n
    • 2017\u5e7412\u6708 \u300c\u30de\u30a6\u30b9\u9855\u5fae\u6388\u7cbe\u6280\u8853\u306b\u95a2\u3059\u308b\u6280\u8853\u7814\u4fee\u300d<\/li>\n
    • 2018\u5e742\u6708 \u300c\u30de\u30a6\u30b9\u9855\u5fae\u6388\u7cbe\u6280\u8853\u306b\u95a2\u3059\u308b\u6280\u8853\u7814\u4fee\u300d<\/li>\n
    • 2018\u5e7410\u6708 \u300c\u30de\u30a6\u30b9\u306e\u7cbe\u5b50\u30fb\u80da\u306e\u51cd\u7d50\u4fdd\u5b58\u65b9\u6cd5\u306b\u95a2\u3059\u308b\u6280\u8853\u7814\u4fee
      \n \uff5e\u52b9\u7387\u7684\u306a\u904e\u6392\u5375\u3001\u82e5\u9f62\u96c4\u3092\u7528\u3044\u305f\u4f53\u5916\u53d7\u7cbe\uff5e\u300d<\/li>\n
    • 2018\u5e7412\u6708 \u300c\u30de\u30a6\u30b9\u306e\u7cbe\u5b50\u30fb\u80da\u306e\u51cd\u7d50\u4fdd\u5b58\u65b9\u6cd5\u306b\u95a2\u3059\u308b\u6280\u8853\u7814\u4fee
      \n \uff5e\u52b9\u7387\u7684\u306a\u904e\u6392\u5375\u3001\u82e5\u9f62\u96c4\u3092\u7528\u3044\u305f\u4f53\u5916\u53d7\u7cbe\uff5e\u300d<\/li>\n
    • 2019\u5e7411\u6708 \u300c\u30de\u30a6\u30b9\u9855\u5fae\u6388\u7cbe\u6280\u8853\u306b\u95a2\u3059\u308b\u6280\u8853\u7814\u4fee\u300d<\/li>\n
    • 2019\u5e7412\u6708 \u300c\u30de\u30a6\u30b9\u9855\u5fae\u6388\u7cbe\u6280\u8853\u306b\u95a2\u3059\u308b\u6280\u8853\u7814\u4fee\u300d<\/li>\n<\/ul>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

      \u30df\u30c3\u30b7\u30e7\u30f3\u3068\u6982\u8981 \u5f53\u7814\u7a76\u5ba4\u3067\u306f\u3001BRC\u304c\u5404\u30ea\u30bd\u30fc\u30b9\u3001\u7279\u306b\u5b9f\u9a13\u52d5\u7269\u304a\u3088\u3073\u5e79\u7d30\u80de\u3092\u3088\u308a\u9ad8\u5ea6\u306a\u30af\u30aa\u30ea\u30c6\u30a3\u306b\u3066\u7dad\u6301\u4f9b\u7d66\u3059\u308b\u305f\u3081\u306b\u5fc5\u8981\u306a\u907a\u4f1d\u95a2\u9023\u6280\u8853\u958b\u767a\u3092\u884c\u3046\u3002 \u307e\u305f\u3001\u3053\u308c\u3089\u306e\u6280\u8853\u304c\u5e83\u304f\u7814\u7a76\u30b3\u30df\u30e5\u30cb\u30c6\u30a3\u306b\u6d3b\u7528\u3055\u308c\u308b\u3079\u304f\u3001\u7814\u4fee\u4e8b\u696d\u3092 […]<\/p>\n","protected":false},"author":7,"featured_media":0,"parent":4271,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","sitemap_hide":"","vkExUnit_sitemap":"","veu_display_promotion_alert":"common","_exclude_from_list_pages":"","_lightning_design_setting":{"layout":"default"},"footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-604","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/kougaku.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/604","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/kougaku.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/kougaku.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/kougaku.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"http:\/\/kougaku.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=604"}],"version-history":[{"count":9,"href":"http:\/\/kougaku.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/604\/revisions"}],"predecessor-version":[{"id":4277,"href":"http:\/\/kougaku.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/604\/revisions\/4277"}],"up":[{"embeddable":true,"href":"http:\/\/kougaku.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/4271"}],"wp:attachment":[{"href":"http:\/\/kougaku.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=604"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}